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目的:优选克疣纳米乳方中主药苦参提取工艺。方法:采用乙醇回流提取苦参总碱,HPLC法测定苦参碱和氧化苦参碱的含量,以苦参碱和氧化苦参碱的总含量为指标、正交试验设计优化提取工艺。结果:乙醇回流提取苦参碱和氧化苦参碱的最佳条件为8倍量70%的乙醇溶液提取3次,每次1.5h。结论:优化提取条件合理、可行。
OBJECTIVE: To optimize the extraction process of Sophora flavescens from Radix Cynocephalus nanoemulsion. Methods: The total alkaloids of Sophora flavescens were extracted by ethanol reflux. The contents of matrine and oxymatrine were determined by HPLC. The total contents of matrine and oxymatrine were used as indexes to optimize the extraction process. Results: The optimum conditions for the extraction of matrine and oxymatrine from ethanol were as follows: the best conditions for the extraction of matrine and oxymatrine were 8 times the amount of 70% ethanol solution three times, each time 1.5h. Conclusion: Optimized extraction conditions are reasonable and feasible.