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As an important plant species with high protein contents, wild soybean (Glycine soja), has drawn much attention and appeared to be useful for the genetic improvement of soybean germplasms. Since temperature is one of the numerous environmental factors affecting the germination of most plants. an experimental study was carried out to determine the effect of temperature on germination of wild soybean(G.soja) seeds. Germination test was conducted by setting up thirty-six constant and alternating temperature regimes, ranging from 5 to 40℃(16 hnight/8 h day).Responses in germination rate to these temperature regimes were then used to construct a quadratic response surface, giving estimated germination rates with confidence intervals at P≤0.05.The results showed that germination capacity was significantly greater while exposed to constant temperatures of 25℃, and under the alternating temperature regime the optimum temperature occurred at the 20/25, 25/25, 25/30℃ regime(16 h/8h)with the amplitude widened from 0 to 5℃.Together with regional monthly climate data, these results could be used to improve and promote the cultivation of wild soybean (G.soja), making it available to develop the location-specific optimum seeding time and to apply weed-control treatments.