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目的探讨胃癌患者血管内皮生长因子A和D(vascular endothelial growth factor-A and-D,VEGF-A andVEGF-D)在胃癌组织、癌旁组织、正常胃组织中的表达及其在胃癌进展中的作用。观察淋巴管内皮细胞podopla-nin的表达,为研究胃癌淋巴道转移的分子机制提供形态学依据。方法应用免疫组织化学SP法检测54例胃癌组织、癌旁组织和12例正常胃组织中VEGF-A、VEGF-D、podoplanin的表达,检测胃癌组织及癌旁组织中的微淋巴管密度(lymphatic microvessel density,LMVD)。结果胃癌癌组织、癌旁组织中VEGF-A和VEGF-D表达阳性率明显高于正常胃组织(P<0.01),其中在淋巴结转移组两者的表达明显高于淋巴结未转移组(P<0.05)。VEGF-A和VEGF-D在胃癌组织中的表达呈正相关(r=0.365,P=0.007),并与淋巴结转移有关(P<0.01)。胃癌癌组织血管内皮podoplanin不染色,淋巴管内皮细胞podoplanin阳性表达,经计数微淋巴管数量,癌组织和癌旁组织LMVD明显高于正常组织,VEGF-A、VEGF-D阳性组LMVD值分别为(9.23±2.49)、(9.29±2.18),较阴性组(7.53±1.81)、(7.44±1.88)显著增高。结论胃癌中VEGF-A与VEGF-D高表达与胃癌淋巴结转移密切相关,podoplanin可选择性的在胃癌组织淋巴管内皮表达,能明确地区分血管及淋巴管成分,可作为一个淋巴管内皮细胞较特异的标记物。由于微淋巴管密度在VEGF-A与VEGF-D阳性表达组癌组织中的表达明显增高,表明淋巴管数量的增加可能与两者协同作用有关。
Objective To investigate the expression of vascular endothelial growth factor-A and VEGF (VEGF-A and VEGF-D) in gastric cancer, paracancerous tissues and normal gastric tissues and their roles in the progression of gastric cancer effect. To observe the expression of podopla-nin in lymphatic endothelial cells and provide morphological evidence for studying the molecular mechanism of lymph node metastasis in gastric cancer. Methods The expressions of VEGF-A, VEGF-D and podoplanin in 54 cases of gastric cancer, adjacent non-cancerous tissues and 12 cases of normal gastric tissues were detected by immunohistochemical SP method. Lymphatic vessel density microvessel density, LMVD). Results The positive rates of VEGF-A and VEGF-D in gastric cancer tissues and adjacent non-cancerous tissues were significantly higher than those in normal gastric tissues (P <0.01). The positive rates of VEGF-A and VEGF-D were significantly higher in patients with lymph node metastasis than those without lymph node metastasis (P < 0.05). There was a positive correlation between VEGF-A and VEGF-D expression in gastric cancer (r = 0.365, P = 0.007), which was correlated with lymph node metastasis (P <0.01). The expression of podoplanin in gastric cancer tissue was not stained and the expression of podoplanin in lymphatic endothelial cells was positive. The number of lymphatic vessels, the LMVD in cancer tissues and paracancer tissues were significantly higher than those in normal tissues. The values of LMVD in VEGF-A and VEGF-D positive groups were (9.23 ± 2.49) and (9.29 ± 2.18) respectively, which were significantly higher than those in the negative group (7.53 ± 1.81 and 7.44 ± 1.88). Conclusion The high expression of VEGF-A and VEGF-D in gastric cancer is closely related to the lymph node metastasis of gastric cancer. The expression of podoplanin in the lymphatic endothelium of gastric cancer can be selectively differentiated and can be used as a lymphatic endothelial cell Specific marker. Because lymphatic vessel density was significantly higher in the VEGF-A and VEGF-D-positive cancers, it was suggested that the increase of lymphatic vessels may be related to the synergistic effect between the two.