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目的:研究5-烯丙基-7-二氟亚甲基白杨素(ADFMChR)对人卵巢癌COC1裸鼠移植瘤生长的影响。方法:建立人卵巢癌COC1裸鼠皮下移植瘤模型,随机分为5组:生理盐水组、顺铂组(2mg/kg)、低剂量ADFMChR组(6mg/kg)、中剂量ADFMChR组(18mg/kg)和高剂量ADFMChR组(54mg/kg),每组4只。观察各组裸鼠移植瘤生长情况,裸鼠体重的变化,检测裸鼠血清谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)、肌酐(CRE)及外周血白细胞计数;PI染色流式细胞术(FCM)测定移植瘤细胞凋亡率。结果:ADFM-ChR有明显抑制移植瘤生长的作用,低剂量组、中剂量组和高剂量组的瘤重抑制率分别为42.86%,62.76%和77.55%。ADFMChR3种剂量组处理裸鼠的外周血白细胞数和血清谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)、肌酐(CRE)与生理盐水对照组的差异均无统计学意义(P>0.05)。PI流式细胞术结果显示ADFMChR诱导移植瘤细胞凋亡,呈剂量依赖性。结论:ADFMChR通过诱导细胞凋亡抑制人卵巢癌裸鼠移植瘤的生长。
OBJECTIVE: To study the effects of 5-allyl-7-difluoromethylenechrysin (ADFMChR) on the growth of human ovarian carcinoma COC1 xenografts in nude mice. Methods: A subcutaneous xenograft model of human ovarian cancer COC1 was established and randomly divided into 5 groups: normal saline group, cisplatin group (2mg / kg), low dose ADFMChR group (6mg / kg), middle dose ADFMChR group (18mg / kg) and high dose ADFMChR group (54 mg / kg), 4 rats in each group. The growth of transplanted tumor in nude mice and the body weight of nude mice were observed. The levels of ALT, CRE and CRE in nude mice were detected. The number of PI-stained flow cytometry The rate of apoptotic tumor cells was determined by FCM. Results: ADFM-ChR significantly inhibited the growth of xenograft tumor. The inhibition rates of tumor weight were 42.86%, 62.76% and 77.55% in low dose group, middle dose group and high dose group respectively. There was no significant difference in peripheral white blood cell count, serum ALT, LDH, creatinine (CRE) and normal saline between ADFMChR3 groups and nude mice (P> 0.05) . Results of PI flow cytometry showed that ADFMChR induced apoptosis of xenografted tumor cells in a dose-dependent manner. Conclusion: ADFMChR can inhibit the growth of transplanted human ovarian cancer xenografts in nude mice by inducing apoptosis.