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玉米单倍体加倍是DH育种中的关键技术环节之一,玉米单倍体雄穗自然加倍能力较低,限制了DH育种技术在商业育种中的大规模推广,对玉米雄穗自然加倍遗传特性的研究将有助于DH技术在育种中的进一步应用。本研究通过对多个不同遗传背景的单倍体雄穗自然加倍能力进行严格统计和差异比较,结果表明,单倍体雄穗育性恢复主要受核基因的控制,不受细胞质基因的调控。诱导率对单倍体雄穗自然加倍能力没有显著影响,自交系来源的DH1经过诱导后的单倍体由于基因型没有变化,其自然加倍能力与自交系相比没有显著提高。
Maize haploid doubling is one of the key technologies in DH breeding. Maize haploid spikes have a natural doubling ability, limiting the large-scale popularization of DH breeding technology in commercial breeding and naturally doubling the genetic characteristics of maize maize Research will help DH further application in breeding. In this study, we strictly complied with the statistics of the natural doubling ability of haploid spawning in different genetic backgrounds. The results showed that the recovery of haploid male fertility was mainly controlled by nuclear genes, but not by the regulation of cytoplasmic genes. Induction rate had no significant effect on natural doubling capacity of haploid males. The natural doubling ability of haploid DH1 induced inbred lines did not significantly increase compared with inbred lines due to no genotype change.