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目的研究布美他尼对Ｃａ２＋、组胺、乙酰胆碱、前列腺素引起豚鼠离体气管平滑肌收缩的影响。方法建立不同浓度Ｂｕｍｅｔ（１０－６、１０－５、１０－４ｍｏｌ·Ｌ－１）孵育时Ｃａ２＋和组胺量效曲线，观察Ｂｕｍｅｔ对Ａｃｈ和ＰＧＦ２α引起气管条收缩的抑制作用。结果Ｂｕｍｅｔ可压低Ｃａ２＋和Ｈｉｓ量效曲线，减活指数分别为３．０８±０．４８和５．６１±０．１３。对Ａｃｈ１０－５ｍｏｌ·Ｌ－１和ＰＧＦ２α５×１０－３ｍｏｌ·Ｌ－１引起的气管平滑肌收缩的ＩＣ５０分别为（５．０９×１０－６±０．１６×１０－６）ｍｏｌ·Ｌ－１和（５．１３×１０－６±０．１７×１０－６）ｍｏｌ·Ｌ－１。结论Ｂｕｍｅｔ可抑制Ｃａ２＋、组胺、乙酰胆碱、前列腺素Ｆ２α引起的豚鼠离体气管平滑肌收缩
Objective To investigate the effects of bumetanide on contraction of isolated tracheal smooth muscle of guinea pigs induced by Ca2 +, histamine, acetylcholine and prostaglandin. Methods The dose-response curve of Ca2 + and histamine in different concentrations of Bumet (10-6, 10-5, 10-4 mol·L-1) was established. The effect of Bumet on tracheal contraction induced by Ach and PGF2α was observed. Results Bumet could depress the Ca2 + and His dose-response curves. The deactivation indices were 3.08 ± 0.48 and 5.61 ± 0.13, respectively. The IC50 of tracheal smooth muscle contraction induced by Ach10-5mol·L-1 and PGF2α5 × 10-3mol·L-1 were (5.09 × 10-6 ± 0.16 × 10-6) mol·L-1 and (5.13 × 10-6 ± 0.17 × 10-6) mol·L-1. Conclusions Bumet inhibits the contraction of tracheal smooth muscle in guinea pigs induced by Ca2 +, histamine, acetylcholine and prostaglandin F2α