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目的检测RECK基因(reversion-inducing-cysteine-rich protein with Kazal motifs)及基质金属蛋白酶-2(MMP-2)在不同卵巢癌细胞株SKOV-3、A2780、HO-8910及正常卵巢上皮细胞中的表达,并探讨其表达差异及其意义。方法应用RT-PCR检测卵巢癌细胞株SKOV-3、A2780、HO-8910及正常卵巢上皮细胞中RECK及MMP-2 m RNA的表达;应用Western blot方法检测不同卵巢细胞株中RECK蛋白的表达。结果卵巢癌细胞株SKOV-3、A2780、HO-8910中RECK m RNA的表达较正常卵巢上皮细胞中明显降低(P<0.01),MMP-2 m RNA表达则明显升高(P<0.01),但三种卵巢癌细胞株中RECK m RNA的表达没有差异(P>0.05);SKOV-3、A2780、HO-8910细胞株中RECK蛋白的表达量较正常卵巢上皮细胞明显降低(P<0.01)。结论 RECK基因在卵巢癌中的表达量明显下降,而MMP-2的表达量明显升高,RECK基因可能通过抑制MMP-2的表达起到抑癌基因的作用。
Objective To detect the expression of reversion-inducing-cysteine-rich protein with Kazal motifs and matrix metalloproteinase-2 (MMP-2) in different ovarian cancer cell lines SKOV-3, A2780, HO-8910 and normal ovarian epithelial cells Expression, and explore the differences in expression and its significance. Methods The expression of RECK and MMP-2 mRNA in ovarian cancer cell lines SKOV-3, A2780, HO-8910 and normal ovarian epithelial cells was detected by RT-PCR. The expression of RECK in different ovarian cell lines was detected by Western blot. Results The expression of RECK m RNA in ovarian cancer cell lines SKOV-3, A2780 and HO-8910 was significantly lower than that in normal ovarian epithelial cells (P <0.01) and MMP-2 mRNA expression was significantly increased (P <0.01) However, the expression of RECK m RNA in the three ovarian cancer cell lines was not different (P> 0.05). The expression of RECK protein in SKOV-3, A2780 and HO-8910 cell lines was significantly lower than that in normal ovarian epithelial cells (P <0.01) . Conclusions The expression of RECK gene in ovarian cancer was significantly decreased, while the expression of MMP-2 was significantly increased. RECK gene may play a role in tumor suppressor gene by inhibiting the expression of MMP-2.