[Objective] To compare the content of bergenin in the Yunnan genus Rodgersia with different species and growing areas. [Method] The content of bergenin in the Yunnan genus Rodgersia with different species and growing areas was determined by HPLC. The chromatographic conditions were as follows: column of Agilent Zorbax XDB-C18 (4.6 mm×150 mm, 5 μm), mobile phase of acetonitrile (A)-0.2% phosphoric acid solution (B) (gradient elution: 0-20 min, 0-20% A), flow rate of 1.0 ml/min, column temperature of 30 ℃, sampling volume of 10 μl and detection wavelength of 275 nm. [Result] Bergenin showed a good linear relationship with absorbance in the range of 3 to 48 μg/ml (r=0.999 6). Bergenin content was the highest in Rodgersia sambucifolia Hemsl. with 5.59%, and the lowest in Rodgersia aesculifolia Batalin with 3.45% among 4 species and 3 varieties of genus Rodgersia. [Conclusion] The bergenin content in the genus Rodgersia was various in different species, and there were obvious differences among different populations from the same species in content. [Objective] To compare the content of bergenin in the Yunnan genus Rodgersia with different species and growing areas. [Method] The content of bergenin in the Yunnan genus Rodgersia with different species and growing areas was determined by HPLC. The chromatographic conditions were as follows : column of Agilent Zorbax XDB-C18 (4.6 mm × 150 mm, 5 μm) mobile phase of acetonitrile -0.2% phosphoric acid solution (B) (gradient elution: 0-20 min, 0-20% A) , flow rate of 1.0 ml / min, column temperature of 30 ° C, sampling volume of 10 μl and detection wavelength of 275 nm. [Result] Bergenin showed a good linear relationship with absorbance in the range of 3 to 48 μg / ml (r = 0.999 6). Bergenin content was the highest in Rodgersia sambucifolia Hemsl. With 5.59%, and the lowest in Rodgersia aesculifolia Batalin with 3.45% among 4 species and 3 varieties of genus Rodgersia. [Conclusion] The bergenin content in the genus Rodgersia was various in different species, and there were obvious differen ces among different populations from the same species in content.