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目的研究临床分离鲍曼不动杆菌耐消毒剂qacE△1-sulI携带情况,探讨其传播机理。方法采用聚合酶链反应(PCR)分析方法,对某医院临床分离鲍曼不动杆菌携带qacE△1-sulI基因状况进行分析。结果从临床分离的92株鲍曼不动杆菌中,检出84株质粒上携带qacE△1-sulI基因,阳性率为91.3%。质粒接合实验结果显示,84株耐消毒剂基因阳性菌株中,有56株菌质粒为可接合质粒,其质粒均存在qacE△1-sulI基因。结论临床分离的鲍曼不动杆菌耐消毒剂基因携带率高,其质粒上携带可接合传递的qacE△1-sulI基因,提示耐消毒剂基因可通过质粒传播。
Objective To study the clinical situation of qacE △ 1-sulI carrier in Acinetobacter baumannii isolates, and to explore the mechanism of its transmission. Methods The status of qacE △ 1-sulI gene in Acinetobacter baumannii isolates from a hospital was analyzed by polymerase chain reaction (PCR). Results Of the 92 Acinetobacter baumannii isolates isolated, 84 isolates of qacE △ 1-sulI gene were detected and the positive rate was 91.3%. The result of plasmid conjugation showed that 56 of the 84 isolates positive for disinfectant genes were jointable plasmids, and qacE △ 1-sulI gene existed in plasmids. Conclusions The clinically isolated Acinetobacter baumannii-resistant disinfectant carries a high rate of gene transfer. The qACE △ 1-sulI gene, which carries on its plasmid, can be transmitted through the plasmid, suggesting that the gene resistant to the disinfectant can be transmitted through the plasmid.