论文部分内容阅读
目的探讨大鼠高浓度矽尘接触过程中是否存在氧化应激反应。方法选40只SPF级Wistar雄性大鼠随机分为4组,即高剂量染尘组(1 000mg/m3)、中剂量染尘组(500mg/m3)、低剂量染尘组(100mg/m3)和对照组,选用自然动式染尘装置每天染尘2h。染尘49d后处死大鼠,测定肺组织匀浆超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)活性及丙二醛(MDA)、还原性谷胱甘肽(GSH)含量。结果长时间、高浓度矽尘接触降低大鼠肺组织的SOD(30.25±0.49)U/ml、T-AOC活性(7.93±0.74)kU/L和GSH(2.34±0.96)g/L含量,同时MDA(5.65±0.13)nmol/ml水平升高,与对照组比较,差异有统计学意义(P<0.01)。结论大鼠高浓度矽尘接触过程早期炎症反应发生可能与机体氧化应急有关。
Objective To investigate the oxidative stress in rats exposed to high concentration of silica dust. Methods Forty SPF Wistar male rats were randomly divided into 4 groups: high dose dyed group (1 000 mg / m 3), middle dose dyed group (500 mg / m 3), low dose dyed group (100 mg / m 3) And the control group, use natural moving dust device daily dust 2h. The rats were sacrificed after 49 days of exposure to dust. The activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) and reducing glutathione . Results The prolonged exposure to high concentrations of silica dust decreased the levels of SOD (30.25 ± 0.49) U / ml and T-AOC activity (7.93 ± 0.74) kU / L and GSH (2.34 ± 0.96) g / The level of MDA (5.65 ± 0.13) nmol / ml increased, compared with the control group, the difference was statistically significant (P <0.01). Conclusions The early inflammatory reaction in rats exposed to high concentration of silica may be related to the body’s oxidative stress.