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对开皇客蚤传播鼠疫菌的机制进行了研究。应用的鼠疫菌株是强毒的195/P 和 Yreka 及减毒的 EV51。跳蚤饲以健康的或感染鼠疫的小白鼠,然后用生物化学和组织学技术来检查。为进行蚤胃和前胃的组织学检查,从活的不经麻醉的蚤、完整地取出这些脏器并立即用福尔马林固定。为作生物化学试验,解剖出来的胃在缓冲液中研磨,制成蚤胃组织匀浆。备作对
The mechanism of transmission of Yersinia pestis was studied. The Yersinia strains used are virulent 195 / P and Yreka and attenuated EV51. Fleas are fed healthy or infected plague mice and then examined using biochemical and histological techniques. For histological examination of the flea’s stomach and the anterior stomach, these organs were removed completely from live, non-anesthetized fleas and immediately fixed with formalin. For biochemical tests, the dissected stomach is ground in a buffer to make a flea stomach tissue homogenate. Ready for right