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背景:要提高含重金属和砷化物矿物药(如朱砂、雄黄)中药制剂的用药安全和排除该类药物的出口障碍,有必要对朱砂、雄黄进行有效性和安全性评价。目前,对复方中朱砂、雄黄的药效作用机制尚不清楚。目的:研究生理、病理状态下安宫牛黄丸中的朱砂、雄黄对机体作用的差异,探讨其药理作用机制。设计:随机对照研究。单位:一所大学的临床药理研究所。材料:实验于2003-01在广州中医药大学临床药理研究所完成。选用第一军医大学实验动物中心提供的体质量为250～300gSD雄性大鼠51只。方法:SD大鼠随机分成6组(8～10只/组):正常组,正常+安宫牛黄散(下简称整方)组(278mg/kg);正常+除去朱砂、雄黄的安宫牛黄散(下简称拆方)组(222.7mg/kg);脑水肿模型组(一侧大鼠颈总动脉注射百日咳杆菌250亿/kg);模型+整方组(造模前1h给药278mg/kg);模型+拆方组(造模前1h给药222.7mg/kg)。一次给药后5h(模型组注菌后4h)采血、制备脑匀浆。主要观察指标:脑组织、血清中乳酸脱氢酶(LDH)总活力,血清中乳酸脱氢酶同工酶LDH1～5百分酶活力。结果:与正常组比较,正常+整方组、正常+拆方组LDH总活力显著升高32.4%～38.4%(P<0.05),LDH1,2百分酶活力均显著升高(P<0.01),LDH4,5百分酶活力下降(P<0.01),但除LDH5外,两组同工酶酶活间无显著差异。与模
Background: To improve the safety of medicines containing Chinese medicines containing heavy metals and arsenide minerals (such as cinnabar and realgar) and to remove barriers to the export of such drugs, it is necessary to evaluate the effectiveness and safety of cinnabar and realgar. At present, the mechanism of action of cinnabar and realgar in the compound is not known. Objective: To study the effects of cinnabar and realgar in Angong Niuhuang Pill on the body under physiological and pathological conditions, and to explore its pharmacological mechanism. Design: Randomized controlled study. Unit: Institute of Clinical Pharmacology at a university. MATERIALS: The experiment was completed at the Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine in 2003-01. Fifty-one male rats weighing 250-300 gSD provided by the Experimental Animal Center of the First Military Medical University were selected. METHODS: Sprague-Dawley rats were randomly divided into 6 groups (8-10/group): normal group, normal + An Gong Niu Huang San (hereinafter abbreviated as whole group) group (278 mg/kg); Normal + Angong Niuhuang with cinnabar and realgar removed. San (hereinafter referred to as disassembled) group (222.7mg/kg); brain edema model group (one side rat common carotid artery injection of B. pertussis 25 billion/kg); model + formula group (administration 1 hour before modeling 278mg/ Kg); model + disassembled group (222.7mg/kg 1h before modeling). Blood was collected and brain homogenate was prepared 5 h after one dose (4 h after injection in the model group). MAIN OUTCOME MEASURES: Total activity of lactate dehydrogenase (LDH) in brain tissue and serum, and LDH 1-5 percent enzyme activity in lactate dehydrogenase isozyme in serum. Results: Compared with the normal group, the LDH total vitality in the normal+twin group and the normal+disassembled group increased significantly by 32.4%-38.4% (P<0.05), and the LDH1,2 percentage enzyme activity increased significantly (P<0.01). ), LDH4, 5 percent enzyme activity decreased (P <0.01), but in addition to LDH5, there was no significant difference between the enzyme activity of the two groups. Modeling