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为了明确我国异位保存的普通野生稻是否能够代表原居群遗传多样性的完整性,利用SSR(简单序列重复)方法对江西省东乡县庵家山和水桃树2个普通野生稻居群的原、异位群体进行了遗传多样性比较研究。结果表明,庵家山居群原、异位保存群体的遗传多样性指数分别为0.5000和0.3555,异位保存群体的遗传多样性只有原位保存群体的71.1%,并且异位保存材料在聚类图中聚集在一起,仅为原位保护材料聚类图中的一个分支;虽然水桃树居群原、异位群体的遗传多样性指数分别为0.4100和0.4577,相差较小,并且原、异位保存材料在聚类图中混合聚类,似乎异位保存群体能够代表原居群的遗传多样性,但异位保存的14份材料在聚类图中有6份相邻的材料聚集在一起,说明异位保存取样时没有考虑野生稻植株的空间分布,属于重复取样,重复取样的比率高达42.9%。将2个分布点原、异位材料合并进行遗传多样性研究,结果与庵家山分布点的研究结果基本一致。据此推测我国保存的普通野生稻资源所包含的遗传变异相对较少,重复频率较高。建议进行普通野生稻遗传资源的再收集,并对已保存的野生稻种质资源进行全面鉴定,剔出重复样品。
In order to determine whether the wild Oryza rufipogon preserved in our country can represent the integrity of the genetic diversity of the original population, SSR (simple sequence repeat) method was used to analyze the genetic diversity of two common Oryza rufipogon populations in Anjiashan Mountain and Peach Trees in Dongxiang County, Jiangxi Province The original and ectopic groups conducted a comparative study of genetic diversity. The results showed that the genetic diversity index of original and ectopic preserved groups in Anjiashan habitat were 0.5000 and 0.3555, respectively. The genetic diversity of ectopic preserved population was only 71.1% of that of in situ preserved individuals, Are clustered together, which is only a branch in the cluster map of in situ conservation materials. Although the genetic diversity indices of the original and ectopic populations of water peach populations were 0.4100 and 0.4577, respectively, the difference was small, and the original and ectopic Preserved Materials Clustered Clustering, It seems that the ectopic population can represent the genetic diversity of the original population, but the 14 materials stored in the ectoplasm have 6 adjacent materials clustered together in the cluster map, This means that the ectopic sampling did not take into account the spatial distribution of wild rice plants, belonging to repeated sampling, sampling rate was as high as 42.9%. The genetic diversity of the original and the ectopic materials at the two distribution points was merged, and the results were basically consistent with the results of the study on the distribution points of Mount Anjiashan. Therefore, it is speculated that the common wild rice resources conserved in China contain relatively few genetic variations and high repetition rates. It is recommended to re-collect common wild rice genetic resources and conduct a thorough appraisal of the preserved wild germplasm resources to eliminate duplicate samples.