作者将FCB_1株恶性疟原虫进行同步化培养,并按Mrema(1982)法用~(35)S-蛋氨酸进行代谢标记,另从培养液中获得自然释放的裂殖子进行放射碘化标记。分离裂殖子的过程中,在完全培养基内加入2V%Tra-sylol(一种广谱的蛋白酶抑制剂)。从完全培养基中获得裂殖子,加入经洗涤新鲜人体红细胞,培养不同时间后染色,观察裂殖子侵入红细胞的情况。将不经过柱、经Con-A柱、蛋白A柱或Cytodex I亲和柱层析的裂 The authors of FCB_1 Plasmodium falciparum were cultured synchronously and metabolically labeled with ~ (35) S-methionine according to the method of Mrema (1982), and naturally released merozoites were obtained from the culture medium for radioiodination. During the separation of merozoites, 2% v Tra-sylol (a broad spectrum of protease inhibitors) was added to complete medium. The merozoites were obtained from the complete culture medium, the fresh human red blood cells were washed and stained at different times to observe the invasion of red blood cells by merozoites. Will not pass through the column, cleaved by Con-A column, Protein A column or Cytodex I affinity column