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以国际合作网络征集的150余份材料,进行农艺性状观察,白叶枯病和稻瘟病的抗性鉴定,同时还进行了耐低磷、低氮筛选,获得大量数据,筛选了一批抗白叶枯病、稻瘟病、耐低磷、耐低氮资源,为分子育种的亲本利用和后代筛选提供参考。以早籼14、紫恢100、M3122为受体,以上述的150余份材料为供体,采用大规模杂交、2￣3次回交、2￣3次自交,培育以早籼14、紫恢100、M3122为背景的近等基因导入系5700多份,并对这些导入系进行抗旱、耐低氮、耐低磷、抗寒性及直链淀粉含量等初步筛选,获得了一批有目标性状的近等基因导入系,为新基因发掘、新品种选育提供重要的材料基础。并选育了3个有苗头的新品系进入省级预试、区试或生产试验,以进一步确定其利用价值。
More than 150 materials collected through international cooperation network were used to evaluate the agronomic traits, bacterial blight and blast resistance. At the same time, we also carried out the screening of low phosphorus and low nitrogen, obtained a large amount of data and screened a batch of anti-white Leaf blight, rice blast, low phosphorus tolerance, low nitrogen tolerance, and provided references for the utilization of progeny of molecular breeding and screening of offspring. Taking early indica 14, purple restore 100 and M3122 as acceptors, more than 150 materials mentioned above were used as donors. Large-scale crossbreeding, 2 ~ 3 backcrossing and 2 ~ Restore more than 100, M3122 as the background of nearly isogenic lines introduced more than 5700 copies, and these import lines for drought resistance, low nitrogen, low phosphorus tolerance, cold resistance and amylose content of the initial screening, obtained a number of targeted The near-isogenic traits of traits provided an important material basis for new gene discovery and breeding of new varieties. And bred three new lines of products into the provincial pre-test, district test or production test to further determine the value of its use.