The dynamic influence of joints in aero-engine rotor systems is investigated in this paper. Firstly, the tangential stiffness and loss factor are obtained from an isolated lap joint setup with dynamic excitation experiments. Also, the influence of the normal contact pressure and the excita-tion level are examined, which revel the uncertainty in joints. Then, the updated Thin Layer Ele-ments (TLEs) method with fitted parameters based on the experiments is established to simulate the dynamic properties of joints on the interface. The response of the rotor subjected to unbalance excitation is calculated, and the results illustrate the effectiveness of the proposed method. Mean-while, using the Chebyshev inclusion function and a direct iteration algorithm, a nonlinear interval analysis method is established to consider the uncertainty of parameters in joints. The accuracy is proved by comparison with results obtained using the Monte-Carlo method. Combined with the updated TLEs, the nonlinear Chebyshev method is successfully applied on a finite model of a rotor. The study shows that substantial attention should be paid to the dynamical design for the joint in rotor systems, the dynamic properties of joints under complex loading and the corresponding inter-val analysis method need to be intensively studied.