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目的观察不同开始时间和不同次数高压氧(HBO)治疗对创伤性脑损伤(TBI)大鼠神经损伤的疗效,明确HBO治疗的时间窗和有效的治疗次数。方法采用Feeney法制备TBI模型,分别在致伤3、6、12、24、48、72 h后开始HBO治疗,观察治疗后大鼠神经行为学、海马结构形态学的变化,确定HBO治疗的有效时间窗和治疗次数。结果治疗组伤后3、6、12 h进行1次HBO治疗,大鼠海马CA2、CA3区锥体细胞形态及神经行为学评分显著改善,细胞丢失显著减少,其中3、6 h的作用最佳(均P<0.01)。而伤后24、48、72 h进行1次HBO治疗,与对照组比较,差异均无统计学意义(均P>0.05)。伤后6 h,进行1、3、5次HBO治疗,各次治疗后在神经行为学、海马结构形态学方面的差异均无统计学意义(均P>0.05)。但伤后24 h,进行1、3、5次HBO治疗后,3、5次治疗效果明显好于1次治疗(均P<0.05)。结论大鼠TBI后6 h内起行HBO治疗效果显著,增加治疗次数有助于增加疗效。
Objective To observe the effects of hyperbaric oxygen (HBO) at different time points on nerve injury in rats with traumatic brain injury (TBI). The time window of HBO treatment and the number of effective therapies were determined. Methods The TBI model was prepared by Feeney method. HBO treatment was started at 3, 6, 12, 24, 48 and 72 h after injury respectively. The changes of neurobehavioral and hippocampal morphological changes were observed after treatment to determine the effectiveness of HBO treatment Time window and treatment times. Results The HBO group treated with HBO at 3, 6 and 12 h after injury showed that the morphological and neurobehavioral scores of hippocampal CA2 and CA3 areas were significantly improved, and the cell loss was significantly reduced, with the best effect at 3 and 6 h (All P <0.01). HBO treatment was performed once 24, 48 and 72 hours after injury, compared with the control group, there was no significant difference (all P> 0.05). 6 h after injury, 1, 3, 5 HBO treatment, after each treatment in neurobehavioral, hippocampal structural morphology differences were not statistically significant (P> 0.05). However, after 1, 3 and 5 HBO treatments, the curative effects of 3 and 5 times were significantly better than those of 1 treatment (all P <0.05) 24 h after injury. Conclusion The HBO treatment is effective within 6 h after TBI in rats. Increasing the number of treatments will help to increase the curative effect.