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为分析簇毛麦6VS导入小麦背景后对品质的影响,利用普通小麦-簇毛麦T6VS.6AL易位系和地方品种辉县红构建的一套小麦F8重组近交家系(RIL)群体,通过分子标记结合白粉病抗性鉴定,筛选出66个包含和94个不包含T6VS.6AL的纯合家系,并分别组成两个亚群体,于2005-2006年分别在江苏南京和河南郑州通过随机区组设计(各3个重复)进行14个品质性状差异比较。结果表明,3对高分子量麦谷蛋白基因在群体及其两个亚群体中均符合1∶1分离。方差分析发现,T6VS.6AL亚群体面粉平均吸水率、面团稳定时间、最大抗延阻力和50 mm处抗延阻力均显著高于非T6VS.6AL亚群体,揭示该易位系对这些性状表现正向效应;T6VS.6AL亚群体籽粒平均容重、面粉峰值黏度和面团弱化度显著低于非T6VS.6AL亚群体,揭示该易位系对这些性状具有负向效应,而T6VS.6AL亚群体面粉平均蛋白质含量、干面筋、湿面筋、出粉率、形成时间、拉伸面积和延伸度与非T6VS.6AL亚群体无显著差异,揭示该易位系对这些性状影响较小。
In order to analyze the effect of introduction of 6VS on wheat quality, the wheat F8 recombinant inbred line (RIL) population constructed by T6VS.6AL translocation line of Triticum aestivum-Tamarix ramosissima and Huixianghong, 66 markers and 94 homozygous lines without T6VS.6AL were screened by molecular markers and resistant to powdery mildew, and they were divided into two subgroups. They were divided into two subgroups in Nanjing, Jiangsu Province and Zhengzhou, Henan Province in 2005-2006 respectively. Group design (3 replicates each) 14 quality traits differences were compared. The results showed that three pairs of high molecular weight glutenin genes in the population and its two sub-populations are in line with the 1: 1 separation. Variance analysis showed that average water absorption, dough stability time, maximum anti-resistance and anti-resistance at 50 mm of T6VS.6AL subpopulation were significantly higher than those of non-T6VS.6AL subunit, revealing that the translocation line was positive for these traits T6VS.6AL subgroup, the average grain weight, the peak viscosity of flour and the degree of dough weakest were significantly lower than those of non-T6VS.6AL subgroup, revealing that the translocation line had a negative effect on these traits, whereas the T6VS.6AL sub-group averaged the average flour There was no significant difference in protein content, dry gluten, wet gluten, flour yield, formation time, tensile area and elongation with non-T6VS.6AL subgroups, suggesting that the translocation line had little effect on these traits.