论文部分内容阅读
目的观察3,6-二羟基黄酮对乳腺癌细胞株MCF-7(雌激素受体阳性ER+)、MDA-MB-231(雌激素受体阴性ER-)、直肠癌细胞株LoVo、DLD-1和前列腺癌细胞株PC3增殖和凋亡的影响。方法MTT法检测3,6-二羟基黄酮对不同肿瘤细胞生长存活的影响,计算半数抑制浓度(IC50);DAPI染色观察3,6-二羟基黄酮对肿瘤细胞生长状态及形态的影响,Annexin V/PI双染流式细胞仪检测细胞凋亡变化。结果增殖检测结果表明,3,6-二羟基黄酮对于MCF-7、MDA-MB-231、LoVo、DLD-1和PC3细胞增殖均表现出显著抑制作用,48hIC50值分别为16.1、15.7、29.8、12.8和17.8μmol/L;细胞形态观察和凋亡检测结果表明,10μmol/L的3,6-二羟基黄酮作用24h,5种肿瘤细胞的生长状态和形态均发生显著变化,大量细胞表现出凋亡或死亡细胞的形态特征,细胞凋亡率均显著升高。结论3,6-二羟基黄酮具有显著的抗肿瘤效应,对于多种肿瘤细胞具有很强的增殖抑制和凋亡诱导作用。
Objective To observe the effect of 3,6-dihydroxyflavone on breast cancer cell line MCF-7 (ERR positive ER+), MDA-MB-231 (estrogen receptor negative ER-), rectal cancer cell line LoVo, DLD-1 The effects of prostate cancer cell line PC3 proliferation and apoptosis. Methods The effect of 3,6-dihydroxyflavone on the growth and survival of different tumor cells was detected by MTT assay. The IC50 was calculated. The effect of 3,6-dihydroxyflavone on the growth and morphology of tumor cells was observed by DAPI staining. Annexin V Apoptosis changes were detected by /PI double staining flow cytometry. Results The results of proliferation assay showed that 3,6-dihydroxyflavone significantly inhibited the proliferation of MCF-7, MDA-MB-231, LoVo, DLD-1, and PC3 cells, and the IC50 values at 48 h were 16.1, 15.7, and 29.8, respectively. 12.8 and 17.8 μmol/L; cell morphology observation and apoptosis detection results showed that 10 μmol/L 3,6-dihydroxyflavone effected 24 h, the growth status and morphology of the five tumor cells were significantly changed, a large number of cells showed withered The morphological characteristics of dead or dead cells, the rate of apoptosis were significantly increased. Conclusion 3,6-dihydroxyflavone has a significant anti-tumor effect, and has a strong proliferation inhibition and apoptosis-inducing effect on a variety of tumor cells.