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在对杂交品种 741杨 (Populusalba (P .davidiana×P .simonii)×P .tomentosa)进行农杆菌介导法转基因的试验中发现了一种快速有效的叶片再生芽的方法。首先叶片外植体在培养基I(MS培养基添加 0 .5mg/LBA和 1 .0mg/L 2 ,4 D)上培养 2～ 3d ,再转移到培养基SH(MSmediumcontaining 2 .0mg/LofBAand 0 .1mg/LofNAA)上培养 1 0d ,然后再转移到培养基II(MSmediumwith 0 .5mg/LofBA)上 ,培养大约 5d之后 86.7%的叶片外植体产生的芽 ,每片叶片外植体 ( 1cm× 1cm)可产生 40～ 5 0个芽。但是 ,如果叶片外植体在培养基I上培养的时间长于 5d ,再依次转移到培养基SH和II上 ,则叶片会产生大量根
A rapid and efficient method of regenerating shoots was found in the Agrobacterium-mediated transgene assay of Populus alba (P. davidiana × P. simonii × P.tomentosa). At first, explants of explants were cultured for 2-3 days in medium I (MS medium supplemented with 0.5 mg / L BA and 1.0 mg / L 2, 4 D) and then transferred to medium SH (MSmediumcontaining 2.0 mg / L of BA 0 .1mg / L of NAA) and then transferred to medium II (MSmedium with 0.5mg / L ofBA). After about 5 days, 86.7% of shoots from leaf explants were cultured, × 1cm) can produce 40 ~ 50 buds. However, if leaf explants were cultured on medium I for more than 5 days and then transferred to medium SH and II in turn, the leaves produced a large number of roots