创伤弧菌溶细胞素(Vvc)是创伤弧菌唯一分泌至细胞外,具备创伤弧菌种属特征性的细胞外蛋白质[1]。其由结构基因VvhA编码,相对分子质量为50 851×103,水溶性好,对热不稳定[2-3]。创伤弧菌溶细胞素的活性和毒素非常强,是创伤弧菌引发细胞、组织损伤的重要毒力因子之一。实验证明,每毫克纯化的Vvc活力可达81 000溶细胞单位(81 000HU/mg)。通过小鼠尾静脉注射毫克级以下水平的Vvc,结果可导致试验 Vibrio vulnificus Vibrio cytolyticus (Vvc) is the only extracellular secretion of Vibrio vulnificus, with Vibrio vulnificus species characteristic extracellular proteins [1]. It is encoded by the structural gene VvhA, with a relative molecular mass of 50 851 × 103, good water solubility and thermal instability [2-3]. Vibrio vulnificus cytolytic cytokines and toxins very strong activity, is Vibrio vulnificus Viral cells, tissue damage is one of the important virulence factors. Experiments show that, per milligram of purified Vvc activity up to 81,000 units of cells (81 000HU / mg). Injection of Vvc at the milligram level through the tail vein of a mouse results in a test