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Poly(pheniothiazine) films were prepared on a porous carbon felt(CF) electrode surface by an electrooxidative polymerization of three phenothiazine derivatives(i.e.,Tthionine(TN),Toluidine Blue(TB) and Methylene Blue(MB)) from 0.1 mol/L phosphate buffer solution(pH 7.0).Among the three phenothiazies,the poly(TB) film-modified CF exhibited an excellent electrocatalytic activity for the oxidation of nicotinamide adenine dinucleotide reduced form(NADH) at +0.2 V vs.Ag/AgCl.The poly(TB) film-modified CF was successfully used as working electrode unit of highly sensitive amperometric flow-through detector for NADH.The peak currents(peak heights) were almost unchanged,irrespective of a carrier flow rate ranging from 2.0 to 4.1 mL/min,resulting in the measurement of NADH(ca.30 samples/hr) at 4.1 mL/min.The peak current responses of NADH showed linear relationship over the concentration range from 1 to 30 μmol/L(sensitivity:0.318 μA/(μmol/L);correlation coefficient:0.997).The lower detection limit was found to be 0.3 μmol/L(S/N = 3).
Poly (pheniothiazine) films were prepared on a porous carbon felt (CF) electrode surface by an electrooxidative polymerization of three phenothiazine derivatives (ie, Tthionine (TN), Toluidine Blue (TB) and Methylene Blue (MB) phosphate buffer solution (pH 7.0). Among the three phenothiazies, the poly (TB) film-modified CF exhibited an excellent electrocatalytic activity for the oxidation of nicotinamide adenine dinucleotide reduced form (NADH) at + 0.2 V vs. Ag / AgCl. poly (TB) film-modified CF was successfully used as working electrode unit of highly sensitive amperometric flow-through detector for NADH. peak currents (peak heights) were almost unchanged, irrespective of a carrier flow rate ranging from 2.0 to 4.1 mL / min, resulting in the measurement of NADH (ca. 30 samples / hr) at 4.1 mL / min.The peak current responses of NADH showed linear relationship over the concentration range from 1 to 30 μmol / L (sensitivity: 0.318 μA / / L); correlation coefficient: 0.997). The lower detectio n limit was found to be 0.3 μmol / L (S / N = 3).