论文部分内容阅读
[Objective] To investigate the optimal conditions of peripheral blood leucocytes phagocytosing Staphylococcus spp. [Method] With Staphylococcus spp. 10 145 as tested materials, phagocytosis activity of chicken′s peripheral blood leucocytes was detected by the method of Wright′s staining. The effects of time, temperature and concentration of Staphylococcus spp. 10 145 on phagocytosis percentages (PP) and phagocytosis index (PI) were optimized by orthogonal test and their morphologic characteristics were observed by microscope. [Result] The concentration of Staphylococcus spp. 10 145 was the primary factor effecting leucocytes phagocytosis index. The optimal phagocytosis conditions were at the temperature of 41 ℃, time of 40 min and concentration of Staphylococcus spp. 10 145 of 5×106/ml. The order of various factors on leucocytes phagocytosis index was the concentration of Staphylococcus spp. 10 145 > time > temperature. [Conclusion] This study provides a more simple and reliable method for detecting leucocytes phagocytosis function.
[Objective] To investigate the optimal conditions of peripheral blood leucocytes phagocytosing Staphylococcus spp. [Method] With Staphylococcus spp. 10 145 as tested materials, phagocytosis activity of chicken’s peripheral blood leucocytes was detected by the method of Wright’s staining. The effects of time, temperature and concentration of Staphylococcus spp. 10 145 on phagocytosis percentages (PP) and phagocytosis index (PI) were optimized by orthogonal test and their morphologic characteristics were observed by microscope. [Result] The concentration of Staphylococcus spp. 10 145 was the primary factor effecting leucocytes phagocytosis index. The optimal phagocytosis conditions were at the temperature of 41 ° C., time of 40 min and concentration of Staphylococcus spp. 10 145 of 5 × 10 6 / ml. The order of various factors on leucocytes phagocytosis index was the concentration of Staphylococcus spp. 10 145> time> temperature. [Conclusion] This study provides a more simple and reliable method for detecting leucocytes phagocytosis function.