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将恶性疟原虫 FCR-3株培养于60×15毫米的培养皿中,培养物总量为5毫升。先将培养物置离心管中,200g 离心5分钟,弃上清液,在沉下的细胞(约0. 5毫升)中加入2. 5毫升5％的 D-山梨醇(0. 274M),混匀,在室温下放5分钟;再离心一次,加入与细胞等容积的含10％人血清的 RPMI1640培养液,再加入培养液和未感染的红细胞,使细胞含量为12. 5％、感染率与起始培养时相仿(通常为0. 1％),重新培养。每天更换培养液1次。在培养后不同的时间采样检查,观察感染率及虫期比例。
Plasmodium falciparum FCR-3 strain was cultured in 60 x 15 mm Petri dishes in a total culture volume of 5 ml. The culture was centrifuged at 200 g for 5 minutes, the supernatant was discarded, and 2. 5 ml of 5% D-sorbitol (0.274 M) was added to the cells (about 0.5 ml) 5%. The infection rate and with the same volume of cells containing 10% human serum RPMI1640 culture medium, and then added to the culture medium and uninfected erythrocytes so that the cell content of 12.5% The initial culture is similar (usually 0.1%), re-culture. Change the culture solution once a day. Samples were taken at different times after cultivation to observe the infection rate and the proportion of worm stages.