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目的:运用血清药理学的方法,探讨复方丹参多元释药微丸对心肌细胞缺氧/复氧损伤的保护作用,评价复方丹参多元释药微丸制剂的有效性。方法:单次灌胃给予市售复方丹参片和复方丹参多元释药微丸(折算生药材2911.14 mg/kg),于给药后0.25,0.5,0.75,1,2,4,8,12 h眼眶取血制备含药血清,并将不同时间点的含药血清作用于心肌细胞,检测心肌细胞的活性。结果:与模型组比较,市售复方丹参片4,8 h的含药血清对缺氧/复氧损伤心肌细胞有促增殖作用,而复方丹参多元释药微丸0.25,0.5,0.75,1,4,8,12 h的含药血清均能够显著促进缺氧/复氧损伤心肌细胞的增殖。结论:复方丹参多元释药微丸对心肌细胞缺氧/复氧损伤具有良好的保护作用,相对于复方丹参片,具有起效快,作用时间长的特点。
OBJECTIVE: To evaluate the protective effect of Fufang Danshen Polydable Drug Delivery Pellets on hypoxia/reoxygenation injury of cardiomyocytes by serum pharmacology, and to evaluate the effectiveness of Fufang Danshen Polypeptides. METHODS: Single-dose intragastric administration of commercially available compound Danshen tablets and compound salvia miltiorrhiza multiple drug release pellets (reported raw herbs 2911.14 mg/kg) was given at the time of 0.25, 0.5, 0.75, 1, 2, 4, 8 and 12 h after administration. Blood was taken from the eyelids to prepare drug-containing serum, and drug-containing serum at different time points was applied to cardiomyocytes to examine the activity of cardiomyocytes. RESULTS: Compared with the model group, the drug-containing serum at the 4 and 8 hours of the compound Danshen tablets had a pro-proliferative effect on the cardiomyocytes injured by hypoxia/reoxygenation, while the compound Danshen polynuclear drug release pellets were 0.25, 0.5, 0.75, and 1, respectively. 4,8,12 h of drug-containing serum can significantly promote the proliferation of hypoxic / reoxygenation injured cardiomyocytes. CONCLUSION: The compound Danshen multi-drug delivery pellets have a good protective effect against myocardial hypoxia/reoxygenation injury. Compared with compound Danshen tablets, it has the characteristics of rapid onset and long duration of action.