论文部分内容阅读
目的检测支原体肺炎患儿免疫功能变化情况及临床意义。方法选取2015年1月-2016年1月本院收治的60例支原体肺炎患儿作为研究组,同期在本院体检的60例健康儿童作为对照组,采用流式细胞术检测2组儿童外周血T淋巴细胞亚群;采用免疫透射比浊法检测2组儿童免疫球蛋白IgG、IgA、IgM;采用ELISA法检测细胞因子γ干扰素(IFN-γ)、白介素4(IL-4)及白介素6(IL-6)表达水平。结果研究组患儿的CD3~+、CD4~+及CD4~+/CD8~+指标值、免疫球蛋白IgA及IgM水平、血清IFN-γ、IL-4及IL-6水平均明显高于对照组,差异有统计学意义(P<0.01),而2组儿童CD8~+指标、IgG水平差异无统计学意义(P>0.05)。结论支原体肺炎患儿存在多种淋巴细胞亚群、免疫球蛋白及细胞因子明显异常,导致自身免疫功能紊乱,检测患儿自身免疫功能状况对支原体肺炎患儿的诊断及治疗具有重要参考价值。
Objective To detect the changes of immune function in children with Mycoplasma pneumonia and its clinical significance. Methods Sixty children with mycoplasma pneumonia admitted in our hospital from January 2015 to January 2016 were selected as study group and 60 healthy children undergoing physical examination in our hospital as control group. Flow cytometry was used to detect peripheral blood T lymphocyte subsets. The immunoglobulin IgG, IgA and IgM of two groups were detected by immunoturbidimetry. The levels of IFN-γ, IL-4 and IL-6 (IL-6) expression levels. Results The levels of CD3 +, CD4 + and CD4 + / CD8 +, immunoglobulin IgA and IgM, serum IFN-γ, IL-4 and IL-6 levels in study group were significantly higher than those in control group Group, the difference was statistically significant (P <0.01), while the two groups of children CD8 ~ + index, IgG level difference was not statistically significant (P> 0.05). Conclusion Mycoplasma pneumonia in children with multiple lymphocyte subsets, immunoglobulins and cytokines significantly abnormal, leading to autoimmune disorders, detection of children with autoimmune conditions in children with mycoplasma pneumonia diagnosis and treatment of important reference value.