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目的通过观察乳腺浸润性导管癌不同病理人乳头状瘤病毒(HPV)高危型HPV18、HPV16感染情况,探讨HPV18、HPV16感染与乳腺浸润性导管癌生成及转化的相关性。方法随机抽取2007年1月-2012年1月128例女性乳腺癌患者送检组织标本,将其分为观察组98例和对照组30例,观察组为乳腺浸润性导管癌患者,对照组为正常乳腺组织;观赛组采用原位杂交方法检测HPV18、HPV16表达情况。结果浸润性导管癌组织中HPV16、HPV18与HPV16和HPV18三者阳性表达率分别为66.33%、71.43%和52.04%,正常乳腺上皮组织中阳性表达率为16.67%、23.33%和10.00%,差异有统计学意义(P<0.05);浸润性导管癌Ⅰ期HPV16、HPV18分子和HPV16与HPV18分子三者阳性表达率分别为43.48%、47.83%和17.39%;Ⅱ期分别为60.00%、63.33%和50.00%;Ⅲ期分别为77.42%、83.87%和67.74%;Ⅳ期分别为92.86%、100.00%和78.57%;不同病理分期间的患者体内三类病毒阳性表达率与Ⅰ期相比,差异有统计学意义(P<0.05),随着癌症病情的恶化,HPV18、HPV16阳性表达增强。结论 HPV18或HPV16感染与乳腺浸润性导管癌的生成和转化存在相关性,且随着乳腺癌病情恶化,HPV18、HPV16表达增强,此发现可为治疗乳腺癌患者提供一种新治疗途径。
Objective To investigate the relationship between HPV18 and HPV16 infection and the generation and transformation of invasive ductal carcinoma in breast by observing the infection of high-risk HPV18 and HPV16 in patients with invasive ductal carcinoma of breast. Methods A total of 128 female patients with breast cancer from January 2007 to January 2012 were randomly divided into observation group (n = 98) and control group (n = 30). The observation group was infiltrating ductal breast cancer patients and the control group was Normal breast tissue. The observation group was detected by in situ hybridization HPV18, HPV16 expression. Results The positive rates of HPV16, HPV18, HPV16 and HPV18 in invasive ductal carcinoma were 66.33%, 71.43% and 52.04%, respectively. The positive expression rates in invasive ductal carcinoma were 16.67%, 23.33% and 10.00% (P <0.05). The positive rates of HPV16, HPV18, HPV16 and HPV18 in invasive ductal carcinomas were 43.48%, 47.83% and 17.39% respectively, and those in stage Ⅱ were 60.00%, 63.33% and 50.00% respectively; the stage Ⅲ were 77.42%, 83.87% and 67.74% respectively; the stage Ⅳ were 92.86%, 100.00% and 78.57% respectively; the positive rate of three kinds of virus in patients with different pathological stages was Statistical significance (P <0.05), with the deterioration of cancer, HPV18, HPV16 positive expression increased. Conclusion HPV18 or HPV16 infection is associated with the formation and transformation of invasive ductal carcinoma of the breast. With the progression of breast cancer, the expression of HPV18 and HPV16 is enhanced. This finding may provide a new therapeutic approach for the treatment of breast cancer.