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作者调查了柏林零售深冷动物性食品的李斯特氏菌的污染情况。检样共122份,做细菌学检验前贮存于-18℃。定性检验参照美国农业部方法(1988年)与食品药物管理局方法(1987年),并用非选择性培养基做平行冷增菌(Gray法)。增菌肉汤(EB1,每升含15 mg吖啶黄素)225 ml。取100 g深冷样品于4℃在冰箱中化冻4小时,粉碎,取25 g加入100 ml EB 1,搅拌1分钟后加入其余125 ml EB 1。于35℃培养24小时后
The authors investigated the contamination of Listeria in the retail, deep-frozen animal food in Berlin. A total of 122 samples were taken and stored at -18 ° C until bacteriological tests were performed. Qualitative tests were made according to the USDA method (1988) and the Food and Drug Administration method (1987), with parallel cold-enrichment (Gray method) using non-selective media. Bacterial broth (EB1, containing 15 mg acridine flavin per liter) 225 ml. 100 g cryogenic samples were thawed in a freezer at 4 ° C for 4 hours, pulverized, 25 g added to 100 ml of EB 1 and stirred for 1 minute before the remaining 125 ml of EB 1 was added. After incubation at 35 ° C for 24 hours