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目的探讨一种新的阿尔茨海默病(AD)大鼠模型与人类AD的相似程度。方法选用正常老年Wistar大鼠,左侧侧脑室注射5μL聚集态的Aβ25-35,通过血清乙酰胆碱酯酶(AChE)的检测和流式细胞仪细胞凋亡测定,比较AD模型大鼠和正常老年大鼠的差异。结果模型组血清AChE活力和脑皮质细胞凋亡百分率均较正常大鼠增大(P<0.05或P<0.01)。结论老年大鼠侧脑室注射聚集态的Aβ25-35的方法可作为制作AD动物模型的方法之一。
Objective To investigate the similarity of a new Alzheimer’s disease (AD) rat model with that of human AD. Methods Normal Wistar rats were selected, 5μL aggregated Aβ25-35 was injected into the left lateral ventricle. The levels of acetylcholinesterase (AChE) and apoptosis were measured by flow cytometry. The AD model rats were compared with normal aged Differences between rats. Results Serum AChE activity and percentage of apoptosis of cerebral cortex cells in model group were higher than those in normal rats (P <0.05 or P <0.01). Conclusion The method of injecting aggregated Aβ25-35 into the lateral ventricle of aged rats can be used as one of the methods for making AD animal model.