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目的探讨银杏叶提取物(金纳多)对氧诱导早产儿视网膜病变(ROP)新生鼠视网膜血管形态和密度的影响。方法将48只SD新生鼠随机分为对照组、模型组、治疗组各16只,模型组与治疗组建立波动氧诱导的新生鼠ROP模型,治疗组于造模第7天开始予以金纳多50mg/(kg·d)腹腔注射7天。各组分别于第14天和第19天随机抽取8只新生鼠,一侧眼球采用视网膜铺片ADP酶染色,观察视网膜血管形态改变,另一侧眼球行组织切片HE染色,观察并计数突破视网膜内界膜的血管内皮细胞核数目。结果视网膜铺片ADP酶染色显示,第14天和第19天时,模型组较对照组血管主干变细、形态异常,而治疗组血管形态和密度较模型组明显好转。HE染色显示模型组第14天和第19天视网膜各层细胞排列紊乱;第19天突破内界膜的细胞核数对照组、模型组、治疗组分别为(5.0±2.1)、(34.6±5.3)、(12.6±3.4),模型组高于对照组和治疗组,差异均有统计学意义(P<0.001)。结论高幅度氧浓度波动可成功构建ROP动物模型。金纳多在氧诱导视网膜病变新生鼠模型中对视网膜发挥保护作用。
Objective To investigate the effects of ginkgo biloba extract (Ginaton) on retinal vascular morphology and density in oxygen-induced retinopathy of prematurity (ROP) in neonatal rats. Methods Forty-eight SD neonatal rats were randomly divided into control group, model group and treatment group with 16 rats in each group. The model rats and the treatment group were given wave oxygen-induced neonatal rat model of ROP. The rats in the treatment group were given Ginaton 50mg / (kg · d) intraperitoneal injection for 7 days. At the 14th day and the 19th day, 8 newborn rats were randomly selected from each group. ADR enzyme staining was used to observe retinal vascular morphology on one eye and HE staining on the other eye. The retinal detachment was observed and counted Endothelial membrane endothelial cell nucleus number. Results ADP enzyme staining of retina showed that on the 14th and 19th days, the vascular trunk of the model group was thinner and the morphology was abnormal than that of the control group. The vascular morphology and density of the treated group were significantly improved compared with the model group. HE staining showed that the cells in the retina were disordered on the 14th and 19th day in the model group, while the number of nuclei in the inner membrane on day 19 was (5.0 ± 2.1), (34.6 ± 5.3) , (12.6 ± 3.4), respectively. The difference between the model group and the control group was statistically significant (P <0.001). Conclusion ROP animal model can be successfully constructed by high amplitude oxygen concentration fluctuation. Ginaton exerts a protective effect on the retina in a neonatal rat model of oxygen-induced retinopathy.