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目的 :研究黄芪液对乳酸盐腹透液致腹膜间皮细胞增殖及损伤作用的影响。方法 :采用胰蛋白酶 -ED TA消化法 ,从人腹膜组织中分离间皮细胞 ,并建立体外人腹膜间皮细胞 (HPMC)培养模型。以MTT法测定HPMC增殖程度 ,采用自动生化分析仪检测 ,培养液中乳酸脱氢酶 (LDH)水平示细胞损伤程度。结果 :2 .5 %乳酸盐腹透液中加用黄芪液 (1mg/ml)与HPMC作用 4 5min ,其培养液中LDH水平较腹透液对照组明显降低(P <0 .0 1) ,HPMC增殖能力明显增强 (P <0 .0 1)。结论 :黄芪液能拮抗乳酸盐腹透液致HPMC的损伤。
Objective : To study the effect of Huangqi liquid on the proliferation and damage of peritoneal mesothelial cells induced by peritoneal lavage fluid. METHODS: The mesothelial cells were isolated from human peritoneal tissues by trypsin-ED TA digestion and a human peritoneal mesothelial cell (HPMC) culture model was established in vitro. The degree of proliferation of HPMC was measured by MTT assay and detected by an automated biochemical analyzer. The level of lactate dehydrogenase (LDH) in the culture fluid showed the extent of cell damage. RESULTS: 2.5 % lactate peritoneal lavage fluid was treated with astragalus solution (1 mg/ml) and HPMC for 45 minutes, and the LDH level in the culture fluid was significantly lower than that in the control group (P < 0.01). HPMC proliferation was significantly increased (P < 0.01). Conclusion: Huangqiye can antagonize the injury of HPMC caused by peritoneal dialysate of lactate.