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试验选取当年生的火力楠的实生苗为试验材料,采用正交试验设计方法L9(34),研究苗木生长各阶段氮、磷、钾的不同配施处理下火力楠的叶子的生长指标(苗高、地径)和生理指标(细胞膜透性、丙二醛(MDA)、叶绿素、可溶性蛋白质含量)的变化规律。结果表明:在不同氮、磷、钾配施处理下,P对苗高、地径起主要作用,N、K次之,在N3P1K3配施下苗高地径的增长均不理想,在N2P2K2配施下增长最好;从N1→N3细胞膜透性呈减小趋势,K1→K3细胞膜透性呈增大趋势,N为主要影响因素,K次之,P最小;影响丙二醛(MDA)因素主到次为N→P→K,N、P、K越多MDA越少;影响叶绿素的因素主到次为N→P→K,N、P、K越多叶绿素含量越少,即在N1P1K3下叶绿素最多,比CK处理的叶绿素含量高32.26%;影响可溶性蛋白质含量因素主到次为N→K→P,K表现不规律,P表现不明显。
In the experiment, the seedlings of Nicotiana benthamiana were used as experimental materials. The orthogonal experiment design method L9 (34) was used to study the growth index of leaves of Nicotiana benthamiana under different treatments of nitrogen, phosphorus and potassium Height, diameter) and physiological index (membrane permeability, malondialdehyde (MDA), chlorophyll, soluble protein content). The results showed that under different treatments of N, P and K, P had the main effect on seedling height and diameter, followed by N and K, and the growth of seedling height under N3P1K3 was not ideal. Under N2P2K2 application The membrane permeability of K1 → K3 cells showed an increasing trend, while N was the main factor, followed by K, P the lowest; the main factor affecting the malondialdehyde (MDA) The next was N → P → K, and the more N, P, K, the less MDA. The main factors affecting chlorophyll were N → P → K, and the more N, P, K, the less chlorophyll, that is under N1P1K3 The content of chlorophyll was 32.26% higher than that of CK. The main factors affecting the content of soluble protein were N → K → P, K was irregular and P was not obvious.