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目的:建立大鼠前庭器官的原代体外培养模型,并通过透射电镜观察前庭上皮细胞在佛波酯(PMA)作用前后的超微结构变化.方法:无菌条件下取出生2d大鼠的椭圆囊上皮,体外培养1~7d;从第2日开始用倒置显微镜观察、照相,试验组加入PMA30min,2h和6h,固定,透射电镜观察.结果:体外培养椭圆囊1wk,12h时可见成纤维细胞沿移植物边缘游出,2d后内皮细胞聚集,呈铺路石样,透射电镜观察细胞无肿胀,胞膜、胞核完整,线粒体嵴无肿胀,纤毛无脱落;PMA各试验组细胞形态正常,核糖体密集,高尔基体较前发达,内质网轻度扩张.结论:大鼠前庭器官体外培养方法可行,为今后进一步研究提供了实验模型.
OBJECTIVE: To establish a primary in vitro model of vestibular organ in rats and observe the ultrastructural changes of vestibular epithelial cells before and after phorbol ester (PMA) by transmission electron microscopy.Methods: Ellipses The epithelial cells were cultured in vitro for 1-7 days.The cells were observed by inverted microscope and photographed on the second day.The cells were fixed in PMA for 30min, 2h and 6h, fixed and observed by transmission electron microscope.Results: After cultured for 1wk in vitro, fibroblasts After 2 days, the endothelial cells were aggregated and showed paving stones. No cell swelling was observed by transmission electron microscopy. The cell membrane and nucleus were intact, the mitochondria cristae were not swollen, and the cilia did not drop off. The cells in PMA test group were normal in morphology and ribose Dense body, more advanced Golgi apparatus, mild endoplasmic reticulum expansion.Conclusion: Rat vestibular organs in vitro culture method feasible for the further study provides an experimental model.