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目的:探讨龟鹿二仙胶对D-半乳糖所致衰老小鼠超氧化物歧化酶(SOD)、丙二醛(MDA)的影响。方法:成年昆明小鼠,雌雄各半,随机分为正常对照组、模型对照组、龟鹿二仙胶组、原方去龟板胶组、原方去鹿角胶组,每组10只。除正常对照组外,其余各组腹腔注射D-半乳糖复制衰老模型,每日2次。正常对照组则腹腔注射等量生理盐水。在造模的同时,正常对照组、模型对照组灌胃等量生理盐水,其余各组灌胃相应实验药物,每日2次。边造模边给药连续进行4周,4周末于腹主动脉采血分离血清并迅速取肝、脑组织进行冰浴匀浆,检测血清及组织匀浆的SOD和MDA含量。结果:模型组小鼠血清和组织匀浆中SOD活力较对照组显著降低、MDA含量显著增高,龟鹿二仙胶组与模型组相比SOD活力升高,MDA含量降低,其它各组比较无统计意义。结论:龟鹿二仙胶对D-半乳糖所致衰老小鼠SOD具有升高作用,对MDA具有降低作用。
Objective: To investigate the effects of Guilu Erxian on superoxide dismutase (SOD) and malondialdehyde (MDA) in aging mice induced by D-galactose. Methods: Adult Kunming mice were randomly divided into normal control group, model control group, Guilu Eryngsian group, untreated Kunrtle group, and untreated group. 10 rabbits in each group. In addition to the normal control group, the remaining groups were injected intraperitoneally D-galactose replication senescence model, 2 times a day. The normal control group was injected intraperitoneally with normal saline. At the same time of modeling, the normal control group and the model control group were given the same amount of normal saline, and the other groups were given the corresponding experimental drugs by gavage twice a day. Serum and tissue homogenate were harvested for 4 weeks and 4 weeks respectively. Blood samples were taken from the abdominal aorta to separate the serum and the liver and brain tissues were rapidly taken for homogenization in ice bath. The content of SOD and MDA in serum and tissue homogenate were determined. Results: Compared with the control group, the activity of SOD in the serum and tissue homogenate of the model group was significantly lower than that of the control group, and the content of MDA was significantly increased. Compared with the model group, the activity of SOD in the Guilu Erxian group increased and the content of MDA decreased Statistical significance. Conclusion: Guilu erxian gum can increase the SOD activity of aging mice induced by D-galactose and decrease the MDA content.