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目的:探讨已受雌、孕激素影响的人子宫内膜细胞.受一氧化氮作用引起细胞生理变化的机制。方法:取人分泌中期子宫内膜。体外原代培养24—28小时,用钙荧光探剂Fluo-3-AM负荷钙离子,采用激光扫描共聚焦显微镜测定加入一氧化氮合酶抑制剂L-NAME后,子宫内膜细胞内游离钙离子[Ca~(2+)]i浓度的动态变化。结果:加入L-NAME后,腺上皮细胞和间质细胞[Ca~(2+)]i立即开始升高,约在加入后400秒上升到高峰,加入后900秒稍有下降,然后继承升高并保持在高水平。同时发现腺上皮细胞和间质细胞对L-NAME的反应不同,其中腺上皮细胞[Ca~(2+)]i升高的幅度显著高于间质细胞。结论:在雌、孕激素作用的基础上,NO通过改变子宫内膜细胞[Ca~(2+)]i进行信号转导,实现其生理效应。
OBJECTIVE: To investigate the mechanism of human endometrial cells that have been affected by estrogen and progesterone and the physiological changes of cells induced by nitric oxide. Methods: Human secrete medium endometrium. Primary cultured in vitro for 24-28 hours, with calcium fluorescence Fluo-3-AM load calcium ions, laser confocal laser scanning microscopy was added nitric oxide synthase inhibitor L-NAME, endometrial intracellular free calcium Dynamic Change of [Ca ~ (2 +)] i Concentration. Results: After adding L-NAME, the glandular epithelial cells and interstitial cells [Ca ~ (2 +)] i began to rise immediately, rising to the peak about 400 seconds after adding, slightly decreasing after 900 seconds after adding, High and stay high. At the same time, the response of glandular epithelial cells and interstitial cells to L-NAME was different. The increase of [Ca ~ (2 +)] i in glandular epithelial cells was significantly higher than that in interstitial cells. Conclusion: Based on the effects of estrogen and progesterone, NO regulates the signal transduction of [Ca ~ (2 +)] i in endometrial cells and realizes its physiological effect.