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目的研究孤啡肽对白血病细胞株HL-60细胞增殖及凋亡的影响。方法选择人急性早幼粒细胞白血病细胞株HL-60为实验模型,应用MTT比色法、形态学观察及流式细胞术(FCM)等方法对孤啡肽诱导其凋亡情况进行研究。结果孤啡肽能显著抑制HL-60细胞增殖,使细胞周期阻滞于G_0/G_1期,细胞凋亡率增加,FCM DNA直方图上G_0/G_1峰前有明显的亚二倍体凋亡峰,表明孤啡肽能明显抑制HL-60细胞的生长,诱导其发生凋亡,且存在时间依赖性,而剂量依赖性不明显。结论孤啡肽对HL-60细胞有增殖抑制和诱导凋亡作用。
Objective To study the effects of orphanin on the proliferation and apoptosis of leukemia cell line HL-60. Methods Human acute promyelocytic leukemia cell line HL-60 was selected as experimental model. The apoptosis of orphanin-induced apoptosis was studied by MTT assay, morphological observation and flow cytometry (FCM). Results Orphanin significantly inhibited the proliferation of HL-60 cells, blocked the cell cycle in G_0 / G_1 phase, increased the apoptosis rate, and obvious sub-diploid apoptotic peak before G_0 / G_1 peak in FCM DNA histogram , Indicating that orphanin peptide can significantly inhibit the growth of HL-60 cells, induce apoptosis, and there is a time-dependent manner, while the dose-dependent manner is not obvious. Conclusion Orphanin can inhibit proliferation and induce apoptosis of HL-60 cells.