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[目的]明确海南芒果蒂腐病(Botryodiplodia theobromae)对8种杀菌剂的抗药性。[方法]以10 mg/L为鉴别剂量进行抗药性初步检测,采用菌丝生长抑制法测定EC50值。[结果]2个苯并咪唑类杀菌剂抗性频率分别为87.5%和90.1%;甾醇脱甲基抑制剂抗性频率在17.1%~74.9%之间。供试菌株对50%多菌灵WP的EC50值在3.23~33 113.11 mg/L之间;对70%甲基硫菌灵WP的EC50值在1.76~11 091.75 mg/L之间,50%多菌灵WP与70%甲基硫菌灵WP之间存在交互抗性。[结论]海南芒果可可球二胞对苯并咪唑类杀菌剂普遍存在抗药性。
[Objective] The research aimed to clarify the resistance of Botryodiplodia theobromae to eight fungicides. [Method] The drug resistance was preliminarily detected with the differential dose of 10 mg / L, and the EC50 value was determined by mycelium growth inhibition. [Result] The resistance frequency of two benzimidazole fungicides were 87.5% and 90.1%, respectively. The resistance frequency of sterol demethylation inhibitor was between 17.1% and 74.9%. EC50 values of the tested strains against 50% carbendazim WP ranged from 3.23 to 33 113.11 mg / L; EC50 values for 70% thiophanate-methyl WP ranged from 1.76 to 11 091.75 mg / L, with more than 50% Mutant WP and 70% thiophanate-methyl WP exist cross-resistance. [Conclusion] Hainan mango cocos balls of benzimidazole fungicides prevalence of resistance.