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目的:建立高效液相色谱(HPLC)法定量检测白芷饮片中欧前胡素和异欧前胡素的含量。方法:色谱柱:Agilent HCC_(18)柱(4.6 mm×150 mm,5μm),以甲醇-水(65∶35)为流动相,柱温25℃,流速1.0mL·min~(-1),检测波长为254 nm。结果:欧前胡素在0.045 2~0.723 2 mg·mL~(-1)范围内线性关系良好(r=0.999 3),异欧前胡素在0.023 8~0.380 8 mg·mL~(-1)范围内线性关系良好(r=0.999 7)。精密度及加样回收率良好。结论:本方法灵敏度高、准确、重复性好,可用于白芷的质量控制。
OBJECTIVE: To establish a HPLC method for the quantitative determination of imperatorin and isoimperatorin in Angelica sinensis Tablets. METHODS: The mobile phase consisted of Agilent HCC 18 column (4.6 mm × 150 mm, 5 μm) with methanol-water (65:35) as the mobile phase at a flow rate of 1.0 mL · min -1 at 25 ℃. The detection wavelength is 254 nm. RESULTS: Imperatorin had a good linearity (r = 0.999 3) in the range of 0.045 2 to 0.723 2 mg · mL -1, and was similar to 0.015 8 to 0.380 8 mg · mL -1 ) Within a good linear relationship (r = 0.999 7). Precision and sample recovery rate is good. Conclusion: The method is sensitive, accurate and reproducible and can be used for the quality control of Angelica dahurica.