论文部分内容阅读
目的:建立以HPLC测定裸花紫珠胶囊中芦丁含量的方法。方法:采用Shim-pack VP-ODS C_(18)(250mm×4.6mm,5μm)柱,以甲醇:水:醋酸=45:55:0.5为流动相,检测波长:257nm,流速1.0ml·min~(-1)。结果:芦丁在0.0303～0.3032μg范围内线性关系良好,r=0.999 8,平均加样回收率为100.1%,RSD为0.9%。结论:本方法操作简便,方法可靠,结果准确、灵敏度高,重复性好,可作为该产品质量控制的方法。
Objective: To establish a HPLC method for the determination of rutin in Nuohua Zizhu capsules. Methods: A Shim-pack VP-ODS C 18 (250mm×4.6mm, 5μm) column was used. The mobile phase was methanol:water:acetic acid=45:55:0.5. The detection wavelength was 257nm and the flow rate was 1.0ml·min~ (-1). RESULTS: The linear relationship between rutin and 0.0303-0.3032 μg was good, r=0.999 8, the average recovery was 100.1%, and the RSD was 0.9%. Conclusion: This method is simple, reliable, accurate, sensitive, and reproducible. It can be used as a method for quality control of the product.