论文部分内容阅读
通过菌种的筛选、培养,获得一株以甲醇为唯一碳源和能源的专性好氧甲醇细菌.与极谱型溶解氧电极结合制成不受乙醇干扰的甲醇细菌电极.以甲醇湿菌体、纤维素、微孔物质、聚乙烯醇(PVA)等为膜材料,采用利于微生物生存和新陈代谢的物理包埋固定化方法制成的微生物膜,不受乙醇等干扰,连续使用3个月,电极响应灵敏度不变.电极的线性响应范围为0.2～9.3mg/L.14次平行测定的相对标准偏差RSD=2.0%,响应时间3～6min,表观米氏常数Km=55.0mg/L.用于测定白酒和工业废水中的甲醇含量,经t检验,电极法测定结果与其它方法相比,以95%置信概率认为无显著性差异.电极法回收率分别为101%～103%,并以本法对车间空气中甲醇含量进行了测定.
Through the selection and culture of the strains, an obligate aerobic methanolic bacterium with methanol as the sole carbon source and energy was obtained, which was combined with the polarographic dissolved oxygen electrode to produce methanol bacteria electrode without ethanol interference. Body, cellulose, microporous material, polyvinyl alcohol (PVA) and other membrane materials, the use of micro-organisms living and metabolism of the physical immobilization method of immobilization of microbial membranes, from ethanol and other interference, continuous use for 3 months , The electrode response sensitivity was unchanged.The linear response range of the electrode was 0.2 ~ 9.3mg / L, the relative standard deviation (RSD) was 2.0%, the response time was 3 ~ 6min, the apparent Michaelis constant Km was 55.0mg / L .For the determination of methanol content in liquor and industrial waste water, the t test showed that there was no significant difference between the results of electrode method and those of other methods with the 95% confidence level.The recoveries of the electrode method were 101% -103% And this method for the determination of methanol in the air workshop.