iRNA活性测定,长期以来,一直是iRNA临床应用和实验室研究中急待解决的问题。目前国际上常用的是微量细胞毒法和无细胞系检测系统,其它还有巨噬细胞或白细胞移动抑制法,国内上一医创建的巨噬细胞电泳法等。然而,上述方法都要求一定的条件:同位素标记的靶细胞、液闪仪:富含mRNA的麦胚翻译测试系统要有~(14)C和~3H标记的亮氨酸等,巨噬细胞电泳也需要特殊的仪器配备。因此,我们选用了巨噬细胞移动抑制法。但是,此法重 The measurement of iRNA activity has long been an urgent problem to be solved in the clinical application and laboratory research of iRNA. At present, the most commonly used methods in the world are micro cytotoxicity and cell-free detection systems. Others include the macrophage or leukocyte migration inhibition method, and the macrophage electrophoresis method created by a doctor in China. However, the above methods all require certain conditions: Isotope-labeled target cells, liquid scintillation devices: mRNA-enriched wheat germ translation test systems have ~14C and ~3H labeled leucine, etc., macrophage electrophoresis Special instrumentation is also required. Therefore, we chose the macrophage migration inhibition method. However, this method