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目的探讨自体骨髓干细胞肝门静脉移植对猕猴急性肝损伤的治疗作用和为人类肝损伤治疗提供依据和方法。方法猕猴10只,随机分为实验组和对照组,每日皮下注射40%CCL40.8ml/kg,连续注射4d,经肝功能生化指标检测及肝穿组织病理学观察确认肝损伤后,分离实验组猴的自体骨髓单个核细胞,按3×108细胞/kg将骨髓细胞植入肝门静脉,植入后60d进行肝功能相关指标检测及组织病理学观察。结果连续注射CCL44d后,血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、球蛋白(GLOB)(P<0.001),而总蛋白(TP)和白蛋白含量无变化(P>0.05),肝组织结构紊乱,大量肝细胞坏死,部分细胞变性,水肿,汇管区及周围有大量炎性细胞浸润。骨髓细胞肝门静脉移植后60d,血清AST、ALT活性和GLOB、BIL含量显著低于对照组(P<0.05),而TP、ALB、LDL含量高于对照组(P<0.05),病理组织学观察结构清楚,而对照组有纤维增生和肝细胞有脂肪变性。结论自体骨髓细胞肝门静脉移植能对猕猴急性肝损伤有明显治疗作用,可改善急性肝损伤猕猴的肝功能及组织结构,提示该方法可能对人类急性肝损伤有一定治疗意义。
Objective To investigate the therapeutic effect of autologous transplantation of hepatic portal vein stem cells on acute liver injury in rhesus monkeys and provide the basis and method for the treatment of human liver injury. Methods 10 macaque monkeys were randomly divided into experimental group and control group. Subcutaneous injection of 40% CCL 40.8ml / kg was injected subcutaneously daily for 4 days. After liver injury and liver biopsy, Group of autologous bone marrow mononuclear cells, bone marrow cells were implanted into the portal vein at 3 × 108 cells / kg, and liver function-related indicators were detected 60 days after the implantation and histopathological observation. Results Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), globulin (GLOB) (P <0.001) and no change of total protein (TP) Tissue structure disorders, a large number of liver cell necrosis, some cell degeneration, edema, portal area and a large number of inflammatory cell infiltration. The level of AST, ALT, GLOB and BIL in bone marrow cells after hepatic portal vein transplantation were significantly lower than those in control group (P <0.05), while the contents of TP, ALB and LDL were higher than those in control group (P <0.05) The structure is clear, while the control group has fibrosis and fatty degeneration of liver cells. CONCLUSION: Hepatic-portal vein grafting of autologous bone marrow cells can significantly improve the acute liver injury of rhesus monkeys and improve the liver function and tissue structure of the rhesus monkeys with acute liver injury. It suggests that this method may have some therapeutic implications for human acute liver injury.