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本文参照Tsunematsu(1960)及Petrov(1974)方法,用弓形虫猪株和人株感染小鼠收集腹腔液,经胰蛋白酶消化,G_3砂芯漏斗过滤,离心,制备较纯的虫体抗原,并用冻干法长期保存。对数批感染小鼠的腹腔液在处理前、后分别进行虫体和细胞计数,用伊文思蓝液染色鉴别死活。并测定虫体的得量、纯度,存活率和回收率。弓形虫猪株和人株感染小鼠腹腔液经上述处理后平均每只获虫体分别为(7.9±1.3)×10~6和(7.5±1.7)×10~6,两者得量无显著差异(t=0.55,P>0.05);虫体纯度在处理前为0.688±0.18, 处理后为0.993±0.004有显著性差异(t=8.125,P<0.01);虫体存活率在胰蛋白酶消化前为99％,消化后为98.25％;从四批感染鼠腹腔液经处理后虫体回收率为87.5％(t=1.33;p>0.05)。
According to Tsunematsu (1960) and Petrov (1974) methods, mice were inoculated with Toxoplasma gondii strain and human strain to collect peritoneal fluid. The peritoneal fluid was collected by trypsin digestion and G 3 sand funnel filtration and centrifuged to prepare the pure antigen. Long-term preservation of freeze-dried. Logarithmic batch of infected peritoneal fluid in mice before and after treatment of parasite and cell count, with Evans Blue staining to identify live and die. The quantity, purity, survival rate and recovery rate of the parasites were determined. Toxoplasma gondii and inoculated mice peritoneal fluid were (7.9 ± 1.3) × 10 ~ 6 and (7.5 ± 1.7) × 10 ~ 6, respectively, with no significant difference (T = 0.55, P> 0.05). The purity of the parasites was 0.688 ± 0.18 before treatment and 0.993 ± 0.004 after treatment (t = 8.125, P <0.01) 99% before and 98.25% after digestion. The recoveries of the parasites from the four groups were 87.5% (t = 1.33; p> 0.05).