用微波法合成了具有发光特性的CDs,以发射波长为447nm的CDs为供体,维生素B2(V B2)为受体,建立了一种以十六烷基三甲基溴化铵(CTAB)为介质的荧光共振能量体系检测VB2含量的方法。结果表明:在pH=7.1时,VB2的浓度与F a/Fd值在1.8×10-8—3.0×10-7mol/L范围内呈线性关系,其线性方程为F a/Fd=0.0766C(×10-8mol/L)+0.5658,相关系数为0.9996,检出限为8.3×10-9mol/L。 CDs with luminescent properties were synthesized by microwave method. CDs with a wavelength of 447nm as donor and vitamin B2 (V B2) as acceptor were synthesized. A novel cetyltrimethylammonium bromide (CTAB) Method for detecting VB2 content in fluorescence resonance energy system of medium. The results showed that there was a linear relationship between the concentration of VB2 and F a / Fd in the range of 1.8 × 10-8-3.0 × 10-7 mol / L at pH = 7.1. The linear equation of VB2 was F a / Fd = 0.0766C × 10-8mol / L) +0.5658, the correlation coefficient was 0.9996, the detection limit was 8.3 × 10-9mol / L.