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哮喘患者气道黏液流变特性易发生变化引发气道栓塞。Fe_2O_3纳米颗粒是一种可经气道输送的药物载体,但其对气道黏液流变特性的影响未见报道。本实验用超声波法分散Fe_2O_3纳米颗粒,采用扫描电镜、原子力显微镜和纳米激光粒度及zeta电位分析仪对其分散结果进行表征;将分散后的Fe_2O_3纳米颗粒加入到模拟哮喘气道黏液中(终浓度分别为0.03、0.3、0.4 mg/m L)。用旋转流变仪进行流动曲线、屈服应力、大幅振荡剪切检测黏液流变学特性的变化。实验结果显示,Fe_2O_3纳米颗粒处理使模拟哮喘气道黏液的零剪切粘度降低,且随着剪切速率的增加,黏液的结缠速度降低;对照组模拟哮喘气道黏液的屈服应力为19.0 Pa,而经Fe_2O_3纳米颗粒处理(0.03、0.3和0.4 mg/m L)的模拟哮喘黏液的屈服应力分别是为17.0、0.99和0.7 Pa;经Fe_2O_3纳米颗粒处理的模拟哮喘气道黏液在大振幅扫描和低频到高频剪切震荡扫描下,其粘弹性模量均出现了较明显的变化。采用光学方法观察气道黏液结构的结果显示,Fe_2O_3纳米颗粒破坏了黏液网状结构。实验结果表明,Fe_2O_3纳米颗粒会改变模拟哮喘气道黏液的流变学特性,本研究的结果可为进一步开发基于Fe_2O_3纳米颗粒的气道黏液解黏剂奠定相应的理论基础。
Airway mucus rheology of patients with asthma is prone to change caused by airway embolism. Fe 2 O 3 nanoparticle is a transdermal delivery drug carrier, but its rheological properties of airway mucus have not been reported. In this experiment, Fe 2 O 3 nanoparticles were dispersed by ultrasonic method, and the dispersion results were characterized by scanning electron microscopy, atomic force microscopy and nano-laser particle size and zeta potential analyzer. The dispersed Fe 2 O 3 nanoparticles were added into the simulated asthma airway mucus Respectively 0.03,0.3,0.4 mg / m L). Flow curves, yield stress, and sharp oscillatory shear were used to determine changes in rheological properties of mucus using a rotating rheometer. The experimental results showed that the Fe2 O3 nanoparticles reduced the zero-shear viscosity of airway mucus of simulated asthma, and the rate of mucous entanglement decreased with the increase of shear rate. The yield stress of airway mucus of simulated asthma control group was 19.0 Pa , While the simulated strain of asthma induced by Fe 2 O 3 nanoparticles (0.03, 0.3 and 0.4 mg / m L) had a yield stress of 17.0, 0.99 and 0.7 Pa, respectively. In the simulated airway mucus treated with Fe 2 O 3 nanoparticles, And low-frequency to high-frequency shear-wave scanning, the viscoelasticity modulus all showed obvious changes. Optical observation of airway mucus structure showed that Fe 2 O 3 nanoparticles destroyed the mucous network structure. The experimental results show that the Fe_2O_3 nanoparticles can change the rheological properties of airway mucus in simulated asthma. The results of this study may provide theoretical basis for further development of airway mucoosolytic adhesives based on Fe_2O_3 nanoparticles.