论文部分内容阅读
妊娠过程中宫颈粘液中前列腺素E(PGE)和前列腺素F_(2α)(PGF_(2α))的浓度决定宫颈的成熟过程。宫颈管内的PGF_(2α),可能使未成熟的宫颈变软,也有看法认为是内源性PG释放的结果。为研究宫颈成熟的机制,本试验收集99例妊娠妇女的宫颈粘液,单独分析抽样前24h内有性交史及有细菌性阴道炎妇女的宫颈粘液。用有短而密的细管的宫颈刷收集宫颈粘液,称出取样前后刷的重量以计算粘液湿重。放此刷于甲基肟盐溶液中,窀温下过夜阻止PGE复合物降解,PGF_(2α)较稳定不形成甲基肟复合物,离心(3000rpm,10min)-70℃存放上清液,放免法测定PGE和PGF_(2α)。结果用t检验比较各组间及孕期不同阶段的均数。
The concentration of prostaglandin E (PGE) and prostaglandin F_ (2α) (PGF_ (2α)) in cervical mucus during pregnancy determines the process of cervical maturation. PGF_ (2α) in the cervical canal may soften the immature cervix, which is also believed to be the result of endogenous PG release. In order to study the mechanism of cervical ripening, we collected cervical mucus from 99 pregnant women and analyzed cervical mucus of women with bacterial vaginosis within 24 hours before sampling. Cervical mucus was collected with a thin, thin tube of cervical brush, and the weight of the brush before and after the sample was weighed to calculate the wet weight of the mucus. Place this brush in methyloxime salt solution, stop the degradation of PGE complex overnight at room temperature, stabilize PGF 2α without forming methoxime complex, and centrifuge at 3000 rpm for 10 min to store the supernatant at -70 ° C Method to determine PGE and PGF 2α. Results t test was used to compare the mean of different stages of pregnancy and between groups.