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为研究在阿糖胞苷（Ara－C）的作用下，基因重组的人白细胞介素3（rh－IL－3）、粒单祖细胞集落刺激因子（rh－GM－CSF）对白血病细胞凋亡的影响，选用了HL－60白血病细胞系，采用DNA电泳、流式细胞仪检测、单克隆抗体C－myc、bcl－2抗原表达的分析以及白血病细胞集落的培养观察方法，观察了0～36h8个不同时相凋亡率与其他指标的变化，表明Ara－C凋亡的作用随药物孵育时间的延长而逐渐增强，凋亡率从0h的1．5％升至36h后的36．4％，而IL－3和GM－CSF能使这种作用明显提高，使凋亡率从7．6％升至19．6％，并能增强Ara－C对白血病细胞的杀灭，引起HL－60细胞C－myc抗原表达下降，而bcl－2抗原表达变化不大，同时发现这两种细胞因子对正常造血细胞影响较小，这为临床上白血病诱导缓解期联用rh－IL－3、rh－GM－CSF和细胞毒药物，提高缓解率，提供了理论依据。
To investigate the effect of Ara-C, recombinant human interleukin 3 (rh-IL-3) and rhg-GM-CSF on leukemia cells withered For the impact of death, the HL-60 leukemia cell line was selected and analyzed using DNA electrophoresis, flow cytometry, analysis of monoclonal antibody C-myc, bcl-2 antigen expression, and culture observation of leukemia cell colonies. Observed 0- The change of apoptotic rate and other indicators at different time points of 36h showed that the effect of Ara-C apoptosis increased with the prolongation of drug incubation time, and the apoptosis rate increased from 1.5% at 0h to 36.4 at 36h. %, while IL-3 and GM-CSF can significantly increase this effect, increasing the apoptotic rate from 7.6% to 19.6% and enhancing the killing of leukemia cells by Ara-C, causing HL- The expression of C-myc antigen was decreased in 60 cells, while the expression of bcl-2 antigen was not changed significantly. It was also found that these two cytokines had little effect on normal hematopoietic cells, which was clinically combined with rh-IL-3 in the period of leukemia induction remission. rh-GM-CSF and cytotoxic drugs improve the remission rate and provide theoretical basis .