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作者报告对12例急性T淋巴细胞白血病患者的新鲜骨髓标本(原始细胞75—100%)的T系白血病祖细胞用三种纯化方案处理的临床前期研究结果。三种方案为:(1)用免疫毒素(IT,1μg/ml)处理(IT是把单克隆抗体抗-CD5和抗-CD 7结合到完整的蓖麻蛋白上,它对白血病T细胞毒性很强而对正常骨髓祖细胞毒性很弱;(2)用4-HC(4-过氧化环磷酰胺,100μg/ml)处理;(3)IT与4-HC联合处理。结果:患者骨髓经方案1体外处理后8/12例残余原始T细胞克隆;经方案2处理后6/9例残余原始T细胞克隆;8例骨髓经方案3处理后清除全部原始细胞克降。IT对正常髓系及红系的造血祖细
The authors report the preclinical results of treatment of T-series leukemic progenitor cells in fresh bone marrow samples (progenitor cells 75-100%) in 12 patients with acute T-lymphocytic leukemia treated with three purification protocols. The three options are: (1) Treatment with immunotoxin (IT, 1 μg/ml) (IT is a combination of the monoclonal antibodies anti-CD5 and anti-CD 7 to intact ricin, which is very toxic to leukemia T cells. Strong but not very toxic to normal myeloid progenitors; (2) Treated with 4-HC (4-peroxycyclophosphamide, 100 μg/ml); (3) Combined treatment with IT and 4-HC. Results: Patients with bone marrow meridian 8 8/12 residual primary T cell clones after in vitro treatment; 6/9 residual primary T cell clones treated by protocol 2; 8 bone marrow treated with protocol 3 to eliminate all primary cell clones. IT vs normal myeloid lineage Erythroid hematopoietic ancestry