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目的:探讨基于传统中药理论色泽分辨黄连药材质量优劣及鉴别药材的方法。方法:采用测色计对黄连药材粉末进行色度测定,表述L*值、a*值、b*值,并用HPLC法测定黄连中小檗碱等6个生物碱的含量,对色度与生物碱含量之间的相关性进行分析。结果:黄连粉末粒度355μm以下,测定径:3 mm;观察视野:10°,F11光下粉末a*值与小檗碱含量(r=0.867,P<0.01)极显著相关;F2光及F7光下粉末a*值与总生物碱含量(r=0.793,P<0.05)显著相关。结论:黄连色度与生物碱含量之间具有相关性,采用测色计测定粉末色度可用于黄连品质的评价。
Objective: To explore the method of distinguishing the quality of Coptis Chinensis based on the traditional Chinese medicine theory of color and distinguish the medicinal materials. Methods: The content of six alkaloids, including berberine in Coptis chinensis Franch, was determined by colorimetry. The values of L *, a *, b * The correlation between content was analyzed. Results: The berberine content (r = 0.867, P <0.01) was significantly correlated with the a * value of F11 under the condition of 355 μm diameter and diameter of 3 mm; The lower powder a * value was significantly correlated with the total alkaloid content (r = 0.793, P <0.05). Conclusion: There is a correlation between the color of Coptis chinensis and the content of alkaloids. The determination of the chroma of powder by colorimeter can be used to evaluate the quality of Coptis chinensis.