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Aim: To evaluate the sperm motility stimulating activity of a sulfono glycolipid (S-ACT-1) isolated from Gelidiellaacerosa, a Sfi Lankan marine red algae. Methods: S-ACT-I, a white amorphous powder was separated from morepolar fractions of the hexane soluble of 1:1 CH_2Cl_2/MeOH extract and subjected to ~1H, ~(13)C NMR and IR Spectroscopyafter reverse phase HPLC for identification. Effects of S-ACT-1 on human sperm motility was assessed in vitro at 10,100 and 1000μg/mL concentrations at 37℃ for 0, 5, 15, 30 and 60 min. Results: S-ACT-1 was identified as aglycolipid sulfate. The lower dose increased the sperm motility slightly, whilst the medium dose significantly increasedthe motility (P < 0.05) from 5 min of incubation reaching a peak at 15 min and the stimulant effect was sustainedthroughout the experimental period. Furthermore, the medium dose rendered 80% of the immotile viable sperm motile.In contrast, the highest dose impaired the sperm motility. The sperm stimulating activity of S-ACT-1 was dos
Aim: To evaluate the sperm motility stimulating activity of a sulfono glycolipid (S-ACT-1) isolated from Gelidiella acerosa, a Sfi Lankan marine red algae. Methods: S-ACT-I, a white amorphous powder was separated from morepolar fractions of the hexane soluble of 1: 1 CH 2 Cl 2 / MeOH extract and subjected to ~ 1H, ~ (13) C NMR and IR Spectroscopy after reverse phase HPLC for identification. Effects of S-ACT-1 on human sperm motility was measured in vitro at 10, 100 and 1000 μg / mL concentrations at 37 ° C for 0, 5, 15, 30 and 60 min. Results: S-ACT-1 was identified as aglycolipid sulfate. 0.05) from 5 min of incubation reaching a peak at 15 min and the stimulant effect was sustainedthroughout the experimental period. Furthermore, the medium dose rendered 80% of the immotile viable sperm motile. In contrast, the highest dose impaired the sperm motility. sperm stimulating activity of S-ACT-1 was dos